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Hi Alami, For most applications, we can purify exosomes in the lab without a flow cabinet or facemask. If you were using them in downstream applications that require sterility (e.g. in cell culture) then you would need to use a flow cabinet. We wouldn't usually use a face mask unless the samples are high risk - we don't handle those types of samples in our labs.

It depends on the dye you are using for your cell cytotoxicity assay.

Hi Luis, Yes, the kit will still be reliable after 6 month's storage

Hi Mohammed, You can see the kits and where to but them here www.cellgs.com/items/exosomes/exospin.html

Hi Dr Prasad. A cell proliferation assay measures any increase in the number of cells. A cytotoxicity assay measures the capacity of cytotoxic compounds to cause cell death, so markers of cell death are measured.

Cell proliferation assays measure cell growth, while cytotoxicity assays assess cell death caused by drugs or other factors. They provide insights into cell health and function, but target different aspects: growth vs. survival.

Hi Matus, Exo-spin is designed to isolate exosomes in the 20-200 nm size range only.

@@CellGS thanks for your reply, but why? Everything bigger than 30nm will be in a void volume anyway so all EVs together if iam not mistaken :). Also when i will the use precipitanion buffer, in the manual it is written that the polymer in the buffer causes that LC-MS downstream method can not be used. Does it mean that it can not be used only immediately after precipitanion or also desalting on a spin column wouldnt help? Thanks :), we need to use it for the LC-MS method.

@@matusjurcik6974 Good question. The reason larger particles >200nm don't come through is because the space between the resin beads isn't big enough. THe 20-200 nm range is because smaller things soak into the cracks in the individual resin beads and larger things just don't get through the gaps between the resin beads efficeintly. So that creates a window of sizes that are purified dependent on the circumference of the resin beads. If you are using LC-MS downstream, we recommend concentrating the sample with other methods such as a micro concentrator. If your starting sample is blood, you won't need to concentrate. You can just add the sample directly to the column. If you have any more questions, please contact tech@cellgs.com to speak with an expert.

@@CellGS Thanks :), ok so it means that there is only Vvoid + Vpore (small contaminants/proteines) and retarded Vvoid alone because some ways can be blocked by bigger (more than200nm) particles right? because bigger particles will stuck ... Also we try to extract EVs from the plasma to screen biomarkers of deseases and we want to use the LC-MS for that.

@@matusjurcik6974 You're rigth. The bigger particles will eventually block the passage of the target exosomes. That's at least in part why there's a limit to the number of times you can reusue the columns. They get progressively slower. If your using plasma, there's no need to concentrate the sample. For screening biomarkers where you're likely to be using large numbers of samples, Exo-Spin 96 is worth looking at.

Exosomes transport molecules between all cells, including immune cells. Cytokines are transported in exosomes between immune cells to help orchestrate the immune system. There are many techniques that allow the study of exosomes both in-vitro and in-vivo. They can be visualized with electron microscopy.

Hi Ahmad, Yes, it is. All cells make and secrete exosomes.

Hi Mohammad, Yes it's very effective for both plasma and sera. There are larger sizes for bigger samples and a 96 well version for purifying large numbers of samples.

Hi Senthilkumar, you can read the protocol here www.cellgs.com/products/oranguand8482--cell-counting-solution.html

Hi I'msadaf, please contact tech@cellgs.com for support

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Please have a look here. www.cellgs.com/exosomes.htm where we show examples of data generated using exosome purified using Exo-spin.

Hi Gungun Barman, Exo-spin kits are optimized for isolating exosomes in this size range. Just follow the standard protocol and you'll achieve this.

Only size based separation cant purify from microvessicles and apoptotic bodies...... Then why you called it exosome? It should be EV for clarification.

@@sharatbarman3352 good point, but I don't think there is a kit on the market that would allow you to separate exosomes from smaller ApBs, MVs or even ectosomes. This is why you ALWAYS need to verify by WB your desired ESCRT biomarkers

Not possible! there is no difference between them at all, what they call viruses are actually exosomes! We have been deceived big times The remarkable resemblance between EVs and viruses has caused quite a few problems in the studies focused on the analysis of EVs released during viral infections. Nowadays, it is an almost impossible mission to separate EVs and viruses by means of canonical vesicle isolation methods, such as differential ultracentrifugation, because they are frequently co-pelleted due to their similar dimension [56,57]. To overcome this problem, different studies have proposed the separation of EVs from virus particles by exploiting their different migration velocity in a density gradient or using the presence of specific markers that distinguish viruses from EVs [56,58,59]. However, to date, a reliable method that can actually guarantee a complete separation does not exist. www.ncbi.nlm.nih.gov/pmc/articles/PMC7291340/

@@yoshicoool6460 what a load of absolute shit.

@@gurpchirp Spoken like a proper moron.

Says 3 replies but none appear. I'm guessing censorship hiding the fact that they are one and the same. Exosomes = so called viruses.

Virus organisms are fakebelieve exosomes are actual things that exist in reality..