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CellGS
Добавлен 25 окт 2011
Technologies for biomedical research
Видео
How to successfully make the switch to synthetic peptide hydrogels
Просмотров 37611 месяцев назад
Technological advances in second generation peptide hydrogels are now producing predictable, reproducible scaffolds which match the best performance of traditional matrices, but more reliably. These hydrogels can be precisely tuned and functionalised to your cells’ specific requirements. Reducing input variability is key to controlling output data quality. Consistent cell culture using traditio...
PODS: Advanced Technology to Stabilize Proteins for In-Vivo Delivery
Просмотров 184Год назад
Proteins can be challenging to work with in-vivo: They need injecting, sometimes to challenging positions such as the brain. And because they degrade quickly, this procedure needs repeating. Whilst this can be distressing for the animals, it can make working with proteins in-vivo practically impossible. PODS is a nature-mimetic technology that stabilizes proteins in micron-scale protein crystal...
Karyotyping iPSCs for chromosomal abnormalities
Просмотров 348Год назад
This webinar, from Cell Guidance Systems’ Cytogenetics team will give both academic and industry researchers an overview of the technique of karyotyping and principles used to determine chromosomal patterns and therefore the genetic stability of the cells. We will also describe the types of abnormalities that are routinely observed, explain which abnormalities are known to affect cell behaviour...
Creating a customizable microenvironment to replicate in vivo properties of tissues
Просмотров 2272 года назад
In this webinar, Dr Natasha Lewis and Dr Dammy Olayanju discuss innovation in 3D cell culture. Complex 3D cell culture models are increasingly used for preclinical and biomedical applications ranging from regenerative medicine to drug discovery and toxicological studies. However, current 3D models are hindered by the inability of the cellular microenvironment to fully provide the molecular cues...
How to embed PODS in PeptiGels
Просмотров 582 года назад
Overview of how to embed PODS® in PeptiGels®. PODS-PeptiGels provide a reproducible and highly controlled environment for 3D cell culture. For more information, visit our website: www.cellgs.com/items/pods-peptigels.html
How to coat plates with PODS for PeptiGel experiments
Просмотров 852 года назад
Overview of how to coat plates with PODS® for PeptiGel® experiments. PODS-PeptiGels provide provide a reproducible and highly controlled environment for 3D cell culture. For more information, visit our website: www.cellgs.com/items/pods-peptigels.html
Co-Isolation of Cytokines and Exosomes: Implications for Immunomodulation Studies
Просмотров 4303 года назад
Exosomes play a vital role in intercellular communication and their immunomodulatory potential has become an important focus in cancer research. In melanoma, tumor-derived exosomes drive immunosuppression within the tumor microenvironment. In this webinar, Dr Shu discusses the possibility that not all cytokines in 'purified' exosome samples are exosome-associated cytokines.
Japanese version: Using the Exo-spin 96 kit to purify exosomes
Просмотров 943 года назад
An overview of the use of Exo-spin 96 to purify exosomes. Exo-spin 96 enables high-throughput purification of exosomes in less than 30 minutes. Up to 500 microliters of each sample can be loaded.
UK Society for Extracellular Vesicles (UKEV) 2020
Просмотров 1753 года назад
Alex Botos, Senior Research Scientist at Cell Guidance Systems, presents as part of the UKEV virtual conference in December 2020. The talk gives an overview of the research tools available as part our exosome range, with a particular focus on the new Exo-spin™ 96. Learn more about the range by visiting: cellgs.com/items/exosomes.html
Macrophages sweep up and ingest PODS cytokine co-crystal nanoparticles
Просмотров 2013 года назад
Macrophages are immune cells which "eat" pathogens such as bacteria. They also take in debris. Normally these ingested particles are rapidly broken down inside the cell into harmless components. PODS co-crystal nanoparticles are broken down slowly to release their cytokine cargo which is then secreted by the macrophages. This provides a potential new mechanism for targeting recombinant cytokine...
Macrophage-mediated localized release of cytokines using PODS
Просмотров 1,4 тыс.3 года назад
PODS protein co-crystal nanoparticles allow the sustained release of cytokines and other proteins from defined locations. Here we show how PODS are readily ingested by macrophages which then allow targetted delivery to tissues the macrophages infiltrate. Learn more at www.cellgs.com/mcpods_yt.htm?E2EPv=1
Macrophages with cytokine nanoparticles looking for trouble
Просмотров 6743 года назад
These are all macrophages, a type of white blood cell and part of our immune system. Most of the cells here have already ingested a nanoparticle co-crystal which may better equip them to treat disease. Read more at www.cellgs.com/mcpods_yt.htm?E2EPv=1 The video is time-lapse capturing movements over a period of 4 hours
Using the Exo-spin 96 kit to purify exosomes
Просмотров 2,4 тыс.3 года назад
An overview of the use of Exo-spin 96 to purify exosomes. Exo-spin 96 enables high-throughput purification of exosomes in less than 30 minutes. Up to 500 microliters of each sample can be loaded.
Enhancing 3D cell culture with growth factor functionalised hydrogels
Просмотров 4943 года назад
Dr Natasha Lewis, Biomaterials Scientist at Cell Guidance Systems, shares her insights into successfully combining PODS® sustained release growth factors with hydrogels
The shocking history of biotechnology
Просмотров 6724 года назад
The shocking history of biotechnology
M0 Macrophages moving across cell culture dish
Просмотров 2,8 тыс.4 года назад
M0 Macrophages moving across cell culture dish
Is exosome purity important to you?
Просмотров 1,6 тыс.4 года назад
Is exosome purity important to you?
How important is a SEC step for extracellular vesicles isolation?
Просмотров 6214 года назад
How important is a SEC step for extracellular vesicles isolation?
Mechanical signaling in stem cell ageing
Просмотров 5834 года назад
Mechanical signaling in stem cell ageing
Cell proliferation and cytotoxicity assay
Просмотров 67 тыс.4 года назад
Cell proliferation and cytotoxicity assay
Improving neuron differentiation outcome with slow-release growth factors
Просмотров 1444 года назад
Improving neuron differentiation outcome with slow-release growth factors
A novel protein technology for improved quality in stem cell differentiation and organoid culture
Просмотров 1174 года назад
A novel protein technology for improved quality in stem cell differentiation and organoid culture
Exosome isolation in less than 10 minutes!
Просмотров 33 тыс.5 лет назад
Exosome isolation in less than 10 minutes!
Extracellular vesicles as a minimally invasive diagnostic source and active metabolic machines
Просмотров 1,5 тыс.5 лет назад
Extracellular vesicles as a minimally invasive diagnostic source and active metabolic machines
Extracellular vesicles; their roles and potential utility in cancer
Просмотров 4,2 тыс.5 лет назад
Extracellular vesicles; their roles and potential utility in cancer
CellGS ZetaView exosome brownian motion
Просмотров 5946 лет назад
CellGS ZetaView exosome brownian motion
There is no potential for anything you barely understand it. Stop trying to make money and do some science. Obviously these were thought to be viruses. Why don't you start there and put a halt to pseudoscience.
:0 wow
Thanks so much.. very helpful ❤ Julia
Thank you
Hbb gene 🧬
Thank you. Good video. Simple enough for this old guy to understand :)
Is it good for treatment of human diseases ?
What is price? What’s made in from
Is the kit suitable for obtaining milk exosome?
Exosomes are pieces of a dead cell, the corona, and it can be used as delivery system for the brain, it can carry nano technology, graphene, for brain / machine interface to the quantum computer, with the help of 5g towers.
I think very good presentation. Thank you for your video.
thank you, amazing video! we need more ladies like this
Fascinante micromundo que esta dentro de nosotros y en vuestros laboratorios.....! Gracias. Y Gracias a Dios su diseñador....
thanks for the video. Normally do you make this process without facemask? I am a MD and I am worry about the posibility of contamination. Thanks again
Hi Alami, For most applications, we can purify exosomes in the lab without a flow cabinet or facemask. If you were using them in downstream applications that require sterility (e.g. in cell culture) then you would need to use a flow cabinet. We wouldn't usually use a face mask unless the samples are high risk - we don't handle those types of samples in our labs.
Great work done, I am PhD Scholar in China, Wish to work with you
Hi can I have more information please how to contact you to order the things for my clinic ?
love it! maybe you can pattern the surface with patches of signaling protein and see if they have favorites :D
From the videos i watched .the color converted from yellow to purple and here is become orange why?
It depends on the dye you are using for your cell cytotoxicity assay.
Hi Julia, can you explain how do you generate your calibration curve?
Hi. Can this kit be used after 6 months (shelf life) and get reliable results?
Hi Luis, Yes, the kit will still be reliable after 6 month's storage
Really nice!!
Thank you for your video. I would like to ask how much of sample volume would be sufficient for exosome isolation (how many ml of urine/serum)? Thank you.
How much this kit and what's agency
Hi Mohammed, You can see the kits and where to but them here www.cellgs.com/items/exosomes/exospin.html
How to contact you ?
Outstanding Mam
WHAT IS THE DIFFRENCE BETWEEN Cell proliferation and cytotoxicity assay
Hi Dr Prasad. A cell proliferation assay measures any increase in the number of cells. A cytotoxicity assay measures the capacity of cytotoxic compounds to cause cell death, so markers of cell death are measured.
Cell proliferation assays measure cell growth, while cytotoxicity assays assess cell death caused by drugs or other factors. They provide insights into cell health and function, but target different aspects: growth vs. survival.
Great job Julia - thanks!
Yeet
I should've been a scientist ... but instead i became a truck driver 😔
Hi Rodrigo, The columns and protocol have been designed to purify exosomes in the size range 30-200 nm. It may be possible to use them to purify other particles in this size range.
Can these be used to purify other samples like secretions, looking for nanosize particles?
Hi :), do you have also something for EV separations at variable sizes? Like 100/200 up to 1000 nm. We need to isolate exosomes as well as microvesicles. Thanks :)
Hi Matus, Exo-spin is designed to isolate exosomes in the 20-200 nm size range only.
@@CellGS thanks for your reply, but why? Everything bigger than 30nm will be in a void volume anyway so all EVs together if iam not mistaken :). Also when i will the use precipitanion buffer, in the manual it is written that the polymer in the buffer causes that LC-MS downstream method can not be used. Does it mean that it can not be used only immediately after precipitanion or also desalting on a spin column wouldnt help? Thanks :), we need to use it for the LC-MS method.
@@matusjurcik6974 Good question. The reason larger particles >200nm don't come through is because the space between the resin beads isn't big enough. THe 20-200 nm range is because smaller things soak into the cracks in the individual resin beads and larger things just don't get through the gaps between the resin beads efficeintly. So that creates a window of sizes that are purified dependent on the circumference of the resin beads. If you are using LC-MS downstream, we recommend concentrating the sample with other methods such as a micro concentrator. If your starting sample is blood, you won't need to concentrate. You can just add the sample directly to the column. If you have any more questions, please contact tech@cellgs.com to speak with an expert.
@@CellGS Thanks :), ok so it means that there is only Vvoid + Vpore (small contaminants/proteines) and retarded Vvoid alone because some ways can be blocked by bigger (more than200nm) particles right? because bigger particles will stuck ... Also we try to extract EVs from the plasma to screen biomarkers of deseases and we want to use the LC-MS for that.
@@matusjurcik6974 You're rigth. The bigger particles will eventually block the passage of the target exosomes. That's at least in part why there's a limit to the number of times you can reusue the columns. They get progressively slower. If your using plasma, there's no need to concentrate the sample. For screening biomarkers where you're likely to be using large numbers of samples, Exo-Spin 96 is worth looking at.
very nice demo and I wish I could see your result of exosome density or yield
ruclips.net/video/PvaLmdE5-DM/видео.html
Do exosomes can help in regeneration of teeth in future dentistry
Chemically do exosomes have anything to do with the immune system other than changes in chemistry per say? I don't know if it would work? Would the use of the trillions per second camera combined with electron microscopes be able to see chemistry in us?
Exosomes transport molecules between all cells, including immune cells. Cytokines are transported in exosomes between immune cells to help orchestrate the immune system. There are many techniques that allow the study of exosomes both in-vitro and in-vivo. They can be visualized with electron microscopy.
it is true exosomes is a nature in our body??
Hi Ahmad, Yes, it is. All cells make and secrete exosomes.
Great. my question is dye is color full so you collected it in 6-7 fraction. While exosomes samples generally colorless, then how we can take them on fraction tube.
It is quite effective video regarding the exosomes isolation. Is it suitable for blood serum and plasma?
Hi Mohammad, Yes it's very effective for both plasma and sera. There are larger sizes for bigger samples and a 96 well version for purifying large numbers of samples.
Concentration in 100microlitres of Sample is?.. Reply...please... thanks
Hi Senthilkumar, you can read the protocol here www.cellgs.com/products/oranguand8482--cell-counting-solution.html
Thanks Jolia Plz tell me how you determine LD50 plz
Hi julia I Tried orangu assay for HCC cell line(Hep3B) , i seeded almost 7500 cells in per well(96 well) and it incubated , but it doesn't work i tried many times but i didn't get result do you have any suggestion?
Hi I'msadaf, please contact tech@cellgs.com for support
Did you do some test to verify the protein contamination in the small vesicles isolation?
Bbmcn s
J l
Nothing on quantified data analysis and results interpretation?
Please have a look here. www.cellgs.com/exosomes.htm where we show examples of data generated using exosome purified using Exo-spin.
Hi Julia, How do you isolate microvesicles and apoptotic bodies in the exosome range (30-200nm), please?
Hi Gungun Barman, Exo-spin kits are optimized for isolating exosomes in this size range. Just follow the standard protocol and you'll achieve this.
Only size based separation cant purify from microvessicles and apoptotic bodies...... Then why you called it exosome? It should be EV for clarification.
@@sharatbarman3352 good point, but I don't think there is a kit on the market that would allow you to separate exosomes from smaller ApBs, MVs or even ectosomes. This is why you ALWAYS need to verify by WB your desired ESCRT biomarkers
exosome
Virus are exossomes
Cool! it was fun to see how this little parasitic like worm was eaten up!
How are exosomes differentiated from viruses?
Not possible! there is no difference between them at all, what they call viruses are actually exosomes! We have been deceived big times The remarkable resemblance between EVs and viruses has caused quite a few problems in the studies focused on the analysis of EVs released during viral infections. Nowadays, it is an almost impossible mission to separate EVs and viruses by means of canonical vesicle isolation methods, such as differential ultracentrifugation, because they are frequently co-pelleted due to their similar dimension [56,57]. To overcome this problem, different studies have proposed the separation of EVs from virus particles by exploiting their different migration velocity in a density gradient or using the presence of specific markers that distinguish viruses from EVs [56,58,59]. However, to date, a reliable method that can actually guarantee a complete separation does not exist. www.ncbi.nlm.nih.gov/pmc/articles/PMC7291340/
@@yoshicoool6460 what a load of absolute shit.
@@gurpchirp Spoken like a proper moron.
Says 3 replies but none appear. I'm guessing censorship hiding the fact that they are one and the same. Exosomes = so called viruses.
Virus organisms are fakebelieve exosomes are actual things that exist in reality..
thank you